What exactly is cell banking?

What is a cell banking system?

Cell banking is the process of maintaining and preserving a population of cells in a culture. Cell banks provide a reliable source of cells for use in laboratory experiments, drug discovery, clinical testing, and more.
The cell banking process includes counting the number of cells present in each sample, measuring their growth rate over time, harvesting them when necessary, and then freezing them for storage. It allows researchers to work with a consistent supply of identical cell lines without having to develop new ones from scratch every time they need them. The resulting banked cell samples are also easier to transport between laboratories than freshly grown batches would be.

Stored cell lines have many applications ranging from basic research into cellular biology to commercial production processes such as developing vaccines or therapeutic drugs. By allowing scientists access to an easily managed stock of living cells, cell banking eliminates much of the difficulty involved with cultivating large populations of various cell types.

What is the purpose of cell banks?

Cell banks are a vital component in the production of safe and effective biopharmaceuticals. They provide an essential source of cells that can be used to create medicines and other cellular products. The primary goal of cell banking is to ensure that cells remain viable over time and maintain their genetic stability through long-term storage.
To achieve this, various services such as cell bank characterization, testing for biosafety, and quality control must be carried out regularly on mammalian cell banks. This helps identify any issues with the cells before use in a manufacturing process or clinical trial. A reference cell bank is also kept so that its contents can be compared against those stored in other banks.

Knowing what is contained within each cell bank allows scientists to assess its suitability for different experiments or applications without needing to culture new batches of cells every time. Cell banking services help ensure that high-quality samples are available quickly and easily whenever needed, allowing researchers and manufacturers access to reliable materials for producing novel treatments or drugs.

What are the steps of cell banking?

Cell banking involves storing master cell banks that are then used to produce production cells which will be further developed into the product itself. The steps involved in cell banking can vary depending on the type of cells being used and the purpose they will serve, but there are some common procedures.

The first step in cell banking is creating a homogenous population of cells, free from any adventitious agents or cross-contamination. This requires careful monitoring of the cell culture conditions including temperature, pH levels, and other factors to ensure optimal growth. Regulatory entities such as the European Medicines Agency (EMA) have specific guidelines for this process.

The next step is to store these cells as a master bank, often in liquid nitrogen so that their age does not significantly change over time due to cell division. Storing them here also serves as a safeguard should anything go wrong with future production batches of cells.

To create new production batches of cells for manufacturing purposes, samples from the master bank are taken and grown up in fresh mediums according to protocol until reaching sufficient density. These production batches may have different requirements than those needed during development such as increased amounts of nutrients or particular concentrations of certain chemicals; all these need to be monitored closely while ensuring adherence to regulatory guidelines.

What are the benefits of cell banking?

he generation of cell banks is an important part of cell line optimization procedures, as it allows for the preservation and storage of cells in a cryopreservation procedure. It provides many benefits to research laboratories and biopharmaceutical companies alike.

Cell banking offers numerous advantages relative to standard cell culture methods:
It enables gene transfection and other processes to be performed on a consistent basis using samples from the same source material. This eliminates the need for researchers to continually create new batches of cells, allowing them to focus their efforts elsewhere.

With a cell banking platform in place, scientists can track the progress of their work by monitoring how each batch performs over time, something which would not be possible without this type of system in place.
Having access to a master cell bank validation process ensures that any human pluripotent stem cells used are subject to current good manufacturing practice (cGMP) compliant process requirements. This helps ensure that these cells remain viable during transportation or long-term storage periods while also preventing contamination or degradation due to external factors such as temperature fluctuations or exposure to light or oxygen.

The ability to monitor certain parameters throughout the maturation antigen process enables scientists to make adjustments if needed, ensuring optimal results when working with different types of cellular substrates.

Where are cell banks stored?

Cell bank repositories can be stored in a variety of ways, depending on the type of cells involved. The storage method must ensure that the cells keep their integrity as well as maintain their genetic variation and cellular characteristics.
When storing a cell bank, it’s important to consider certain factors like how many cells per vial or tube are needed, along with any cytopathic effects (CPE) associated with the collection. It’s also necessary to determine the cell confluence required for growth and proliferation profile, as this allows us to know if we have enough biological products from each vial or tube.

Proper temperature control, light exposure levels, and other environmental conditions must be taken into account when deciding where to store cell banks. This ensures they remain viable for future use (gene therapy, stem cell-based interventions, etc.).

Cell banking services

Cell banking offers many advantages over traditional laboratory methods by providing precise control over environmental parameters such as temperature and humidity, ensuring maximum efficiency and success rate when handling sensitive samples like those found in biotechnological applications. It also provides improved traceability due to rigorous tracking procedures, making this process ideal for research projects requiring high-quality data management systems.

At NorthX Biologics, we have the technology and expertise to manufacture GMP-grade Master and Working cell banks, providing you with the cells you need to make your next project a success. Whether you need cell banking services as part of your project or the management of your entire production process, working alongside NorthX Biologics will allow you to leverage the best cell banking systems.

About NorthX Biologics AB

NorthX Biologics is a CDMO and Innovation Hub in Advanced Biologics, with +30 years of GMP production experience. The team provides process development and GMP manufacturing services with expertise in plasmid DNA, mRNA, proteins, cells and other advanced biologics. Headquartered in the heart of Sweden, the team serves customers worldwide and in 2021 was recognized as a national innovation hub for advanced therapeutics and vaccines. NorthX has the ambition to become a leading cell and gene therapy manufacturer and partner of choice for innovative drug development companies.

For more news, follow us on