Plasmid DNA Production
We are experts in the production of plasmid DNA and can support your project from pre-clinical through to commercial manufacture. We offer plasmid DNA to the right quality at the right time, no matter if you are working with DNA vaccines, viral vector gene therapies, mRNA or any other modalities.
Contact us to know more about plasmid DNA by NXBio
Applications of NXBio's plasmid DNA production
Our high-quality plasmid DNA services are essential for various applications in research, clinical, and commercial fields. Some of the key applications include:
AAV/Lenti vector manufacturing
Adeno-associated virus (AAV) and lentiviral vectors are widely used for gene delivery in gene therapy applications. NXBio's plasmid DNA services provide high-quality, endotoxin-free plasmid DNA required for the production of these viral vectors. Our expertise ensures efficient and reliable large-scale plasmid DNA production, which is a critical component for successful AAV and lentiviral vector manufacturing.
Non-viral gene therapy
Non-viral gene delivery methods, such as electroporation and lipofection, require high-quality plasmid DNA to achieve efficient transfection and gene expression. NXBio's plasmid DNA services offer purified, endotoxin-free plasmid DNA that can be used for various non-viral gene therapy applications, ensuring optimal gene delivery and therapeutic efficacy.
DNA vaccines
DNA vaccines represent a promising approach to combat various infectious diseases and stimulate immune responses against cancer. Our plasmid DNA services provide high-quality, endotoxin-free plasmid DNA that can be used as the backbone for DNA vaccine development. By delivering consistent and reliable plasmid DNA, NXBio supports the advancement of DNA vaccine research and development.
mRNA manufacturing
The production of messenger RNA (mRNA) for therapeutic applications, such as mRNA vaccines and gene therapies, requires the use of plasmid DNA templates. NXBio's plasmid DNA services offer high-quality, endotoxin-free plasmid DNA that can be used as the starting material for in vitro transcription and subsequent mRNA manufacturing. Our plasmid DNA services ensure a consistent and reliable supply of plasmid DNA templates for your mRNA production needs.
With NXBio's plasmid DNA services, you can be confident in the quality and reliability of the plasmid DNA you receive for your research and clinical applications. Trust our expertise to support your work in gene therapy, vaccine development, and other cutting-edge fields.
We offer a range of plasmid qualities, qualities meeting the specific needs for each development phase from early R&D to full GMP. We are using aligned processes ensuring scalability from small to large scale.
GMP grade plasmid DNA by NXBio
NXBio is dedicated to providing high-quality GMP grade plasmid DNA for clinical and commercial applications. Our GMP grade plasmid is manufactured according to full Good Manufacturing Practice (GMP) standards in qualified clean rooms, ensuring the highest level of quality and safety for use in applications such as DNA vaccines, direct-delivered gene therapy, and late-phase viral vector products.
Key features of our GMP grade plasmid DNA
Fully single-use process equipment
We utilize single-use process equipment to minimize the risk of cross-contamination and ensure the highest level of purity and consistency for our GMP grade plasmid DNA.
Fully traceable non-animal origin raw materials
Our manufacturing process exclusively uses non-animal origin raw materials, which are fully traceable to ensure the safety and ethical sourcing of our plasmid DNA products.
RNase-free process
Our manufacturing process is designed to prevent RNase contamination, ensuring the integrity and quality of our GMP grade plasmid DNA.
Drug substance quality
NXBio's GMP grade plasmid DNA meets the stringent quality requirements for drug substance, ensuring the safety and efficacy of the final therapeutic product.
GMP level analytics and certificate of analysis (CoA)
We perform comprehensive GMP level analytics on our plasmid DNA products and provide a detailed Certificate of Analysis (CoA) for quality assurance and regulatory compliance.
EU release via on-site qualified person (QP)
Our in-house Qualified Person (QP) reviews and releases the GMP grade plasmid DNA products, ensuring that they meet all relevant European Union (EU) regulatory requirements for clinical and commercial use.
Clinical and commercial scale
NXBio offers GMP grade plasmid DNA production at both clinical and commercial scales, ensuring reliable supply for your research and product development needs.
At NXBio, we understand the critical role that high-quality GMP grade plasmid DNA plays in the development and production of gene therapies, vaccines, and other advanced therapies.
Trust our expertise and commitment to quality for your clinical and commercial plasmid DNA requirements and let us know if you have any questions.
At NXBio, we understand the critical role that high-quality GMP grade plasmid DNA plays in the development and production of gene therapies, vaccines, and other advanced therapies.
High quality grade plasmid DNA manufacturing by NXBio
NXBio offers High Quality grade plasmid DNA for preclinical applications in vitro or in vivo, including toxicology studies. Our High Quality plasmid DNA is manufactured in accordance with regulatory expectations for critical starting material for cell and gene therapies, making it suitable for the production of viral vectors for clinical use. By utilizing our defined platform technology, we provide a fast turnaround from the start to the delivery of the plasmid.
Key features of our high quality grade plasmid DNA manufacturing
Critical starting material for cell and gene therapies
Our High Quality plasmid DNA is a crucial starting material for various cell and gene therapy applications, ensuring the reliability and safety of your research and development efforts.
mRNA template
Our High Quality plasmid DNA can be used as a template for mRNA production, supporting the development of mRNA-based therapeutics and vaccines.
Preclinical applications in vitro or in vivo, including toxicology studies
Our High Quality grade plasmid DNA is suitable for a wide range of preclinical applications, such as in vitro or in vivo studies, and toxicology assessments, ensuring that your research is supported by the highest quality materials.
Aligned high quality level analytics
We perform comprehensive analytics on our High Quality plasmid DNA products to ensure their quality, purity, and consistency throughout the manufacturing process.
Segregated laboratories
Our High Quality plasmid DNA is produced in segregated laboratories, minimizing the risk of cross-contamination and ensuring the highest level of product integrity.
Trust NXBio for your High Quality grade plasmid DNA needs, supporting your preclinical research and development efforts with reliable and high-quality materials.
Research grade plasmid DNA by NXBio
NXBio offers Research Grade plasmid DNA for pre-clinical in vitro or in vivo applications where a fast turnaround of affordable, high-quality plasmid is required. Our Research Grade plasmid DNA is produced using platform technology aligned with High Quality (HQ) and Good Manufacturing Practice (GMP) grade plasmids, ensuring consistency and reliability for your pre-clinical research needs.
Key features of our research grade Plasmid DNA
For pre-clinical research
Our Research Grade plasmid DNA is specifically designed for pre-clinical research applications, providing a cost-effective solution without compromising on quality.
Platform technology aligned with HQ/GMP grade
We utilize a manufacturing platform that aligns with our HQ and GMP grade plasmid DNA services, ensuring consistent quality and performance across all grades of plasmid DNA.
Cost-effective and fast turnaround
Our Research Grade plasmid DNA services are designed to be both cost-effective and efficient, ensuring that you receive high-quality plasmid DNA with a fast turnaround time to support your research timeline.
Provided with certificate of testing
We provide a Certificate of Testing with our Research Grade plasmid DNA to confirm its quality and suitability for your pre-clinical research applications.
Flexible basic analytic panel
Our Research Grade plasmid DNA services include a flexible basic analytic panel, allowing you to customize the analytics based on your specific project requirements.
Choose NXBio for your Research Grade plasmid DNA needs, and trust our expertise to deliver affordable, high-quality plasmid DNA with a fast turnaround time for your pre-clinical research applications.
Contact us if you have any questions about our plasmid DNA manufacturing services!
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Learn more about plasmid DNA by NXBio
What is plasmid DNA?
Plasmid DNA refers to small, circular, double-stranded DNA molecules found in bacterial cells. Plasmids carry antibiotic resistance genes or other functional genes and are widely used in molecular biology applications, such as gene therapy and cell therapy.
How does NXBio ensure plasmid DNA purification and plasmid DNA isolation?
NXBio uses the alkaline lysis method for the isolation of plasmid DNA from bacterial cells, followed by purification steps, including anion-exchange chromatography and ethanol precipitation. These methods ensure high-quality plasmid DNA with minimal contamination.
What are the applications of plasmid DNA?
Plasmid DNA has a wide range of applications, including gene therapy, cell therapy, DNA sequencing, molecular biology research, and clinical applications such as DNA vaccines and mRNA manufacturing.
How do I know the plasmid yield and quality?
NXBio performs comprehensive analysis, including UV analysis, AGE analysis, and restriction enzyme digestion, to determine the concentration and purity of plasmid DNA. We provide a Certificate of Analysis (CoA) that includes details about the plasmid yield, concentration, and quality.
What bacterial strains does NXBio use for plasmid DNA production?
NXBio commonly uses E. coli strains, such as E. coli DH5α, for plasmid DNA production. These strains are known for their high plasmid yields and compatibility with a wide range of resistance plasmids.
Does NXBio provide endotoxin-free plasmid DNA?
Yes, NXBio offers endotoxin-free plasmid DNA for sensitive applications, such as gene therapy and clinical applications. Our purification of plasmid DNA methods, including tangential flow filtration and chromatography steps, help to achieve endotoxin-free plasmid DNA.
Can NXBio support different plasmid DNA scales and grades?
Yes, NXBio offers a variety of plasmid DNA services, including Research Grade, High Quality Grade, and GMP Grade plasmid DNA, to accommodate different scales and applications, from pre-clinical research to clinical and commercial use.
How does NXBio ensure the quality and consistency of plasmid DNA?
NXBio utilizes single-use or reused process equipment, segregated laboratories, and fully traceable non-animal origin raw materials to maintain the quality and consistency of our plasmid DNA products. Our manufacturing processes are aligned with regulatory standards, and our plasmid DNA is provided with a Certificate of Testing.